Stanford University. Millegen Biotech and University of Copenhagen has collaborated supported by Juvenile Diabetes Research Foundation in the development of a new recombinant bioluminescent fusion protein scFv-Gluc, with the aim of testing the new format for in vivo noninvasive biolumeniscent imaging (BLI) of beta-cells in whole animals and pancreas tissue. The first fusion protein is […]
The IC2 monoclonal autoantibody and its biotinylated, HRP, FITC and PE conjugated formats are planned to be commercial available from September 1st, 2011 after stability and quality control. Antibody-online.com will on commission contract with ImmunoSigns do both marketing and sale, but the products will also be directly available from ImmunoSigns in Copenhagen directly from this […]
Using 125-I iodinated tracers with both native IC2 – the IgM format -, and the pepsin digested F(ab’)2 and Fab fragments, plus in comparison two other beta-cell reactive monoclonals the mouse IgM A2B5, the mouse IgG K14D10, and an isotype Rat IgM control, its has been confirmed in biodistribution studies in both mice and rats, […]
It has already been know for many year the the IC2 monoclonal autoantibodies sees epitipes located in socalled lipid rafts on the cell surface of the beta-cells. Studies by confocal laser fluorescence microscopy will now be supplement with studies aiming to characterize the exact epitope for the unique beta-cell specific autoantibodies.
By action from September 1st 2010, the basic and clinically aiming research associated with the monoclonal autoantibody IC2 and its unique autoantigen specific on the surface of the pancreatic beta-cell, will move from University of Copenhagen, Department of Biomedical Sciences, to the new and modern Copenhagen Biocenter, and be part of The Bartholin Institute, which […]
A large scale production of the native IgM IC2 monoclonal autoantibody is under execution on contact with the Chech Rebublicbiotech company EXBIO.
The European Commission initiative – the EU-FASILIS Programme – has funded ImmunoSigns with a Voucher to collaborate with Modiquest in Nijmegen for an expanded pilot production of recombinant and/or native fragmented IC2 monoclonal autoantibodies.
The IC2 monoclonal autoantibody has now been DNA cloned and expressed in various new formats including a chimeric humanized IgG format – this work has been done with support from International Juvenile Diabetes Research Foundation in the period 2008-10.
EU-”People” Research Training Program in Biotech and Bioethics aim to use IC2 non-invasive imaging of beta-cells mass in studies of induced chemeric immuno tolerence in animal moels of diabetes.
Preliminary data with affinity crosslinking and flow cytometry indicate that proteins of the SNARE-cascade regulating the insulin excretion from beta-cells could be associated with the IC2 specific targeted epitope.